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NSE Antibody

CAT: 0800-V7210SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V7210SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a protein of about 50kDa, which is identified as gamma-enolase/neuron specific enolase/enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL
UniProt
P09104
Host
Mouse
Reactivity
Human
Immunogen
Amino acids 416-433 of human Neuron-specific Enolase were used as the immunogen for this NSE antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
NSEL1-1
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the NSE antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This NSE antibody is available for research use only.
Storage Conditions
Store the NSE antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance.
Location
Cytoplasmic
Image Legend
IHC testing of FFPE human pancreas with NSE antibody (clone NSEL1-1) . Staining of FFPE tissue requires boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

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