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S100A9 Antibody

CAT: 0800-V7141-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V7141-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
S100A9, also known as Calgranulin B or MRP14 (and Calprotectin L1H), is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. [UniProt]
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT (1)
UniProt
P06702
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the S100A9 antibody.
Clonality
Monoclonal
Isotype
IgM k
Clone
PS1A9-1
Applications
IHC-P
Purity
PEG precipitation
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the S100A9 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This S100A9 antibody is available for research use only.
Storage Conditions
Store the S100A9 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Titering of the S100A9 antibody may be required for optimal performance.1. Not suitable for frozen tissue testing.
Location
Cytoplasmic
Image Legend
IHC testing of FFPE human tonsil with S100A9 antibody (clone PS1A9-1) . Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

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