FOXP3 Antibody

• Description:

  The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. FOXP3 is essential for normal immune homeostasis. It represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  An N-terminal recombinant protein fragment was used as the immunogen for this FOXP3 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  FHBP3-1

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the FOXP3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This FOXP3 antibody is available for research use only.

• Storage Conditions:

  Store the FOXP3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Titering of the FOXP3 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• CAS Number:

  9007-83-4

• Location:

  Predominantly nuclear, some cytoplasmic

• Image Legend:

  IHC analysis of FOXP3 antibody (clone FHBP3-1) and human tonsil tissue. Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min.