SUMO-2 Antibody

• Description:

  The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, 2 and 3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, 2 and 3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-2 and 3 contribute to a greater percentage of protein modification than does SUMO-1 and unlike SUMO-1, they can form polymeric chains.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

• UniProt:

  P61956

• Host:

  Mouse

• Reactivity:

  Human, Rat

• Immunogen:

  Recombinant human protein was used as the immunogen for the SUMO-2 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  S2MT-1

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the SUMO-2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This SUMO-2 antibody is available for research use only.

• Storage Conditions:

  Store the SUMO-2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Titering of the SUMO-2 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• CAS Number:

  9007-83-4

• Location:

  Nuclear

• Image Legend:

  IHC testing of formalin-fixed, paraffin-embedded human tonsil with SUMO-2 antibody (clone S2MT-1) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.