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Calponin Antibody

CAT: 0800-V7050-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V7050-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
UniProt
P51911
Host
Mouse
Reactivity
Human, Rat
Immunogen
Recombinant human protein was used as the immunogen for the Calponin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
CLPN5-1
Applications
IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Calponin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Calponin antibody is available for research use only.
Storage Conditions
Store the Calponin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Calponin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Location
Cytoplasmic
Image Legend
Immunofluorescent staining of PFA-fixed human K562 cells with Calponin antibody (green, clone CLPN5-1) and Reddot nuclear stain (red) .

UniProtKB · P51911

Calponin-1

CNN1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P51911
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CNN1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B2R868, B4DUX6, O00638, Q15416, Q8IY93, Q99438
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Molecular function

Actin-bindingCalmodulin-binding

Coding sequence diversity

Alternative splicing

PTM

Phosphoprotein

Domain

Repeat

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