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EGFR Antibody

CAT: 0800-V7040-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V7040-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. [UniProt]
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells in 0.1ml, Immunofluorescence: 1-2 µg/mL
UniProt
P00533
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for this EGFR antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
ERB59-1
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the EGFR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This EGFR antibody is available for research use only.
Storage Conditions
Store the EGFR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EGFR antibody to be titered up or down for optimal performance.
Location
Cell surface
Image Legend
Immunofluorescent testing of A431 cells and Alexa Fluor conjugated EGFR antibody (green, clone ERB59-1) with DAPI nuclear stain (blue) .

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