Progesterone Receptor Antibody

• Description:

  Progesterone receptor is expressed as two major isoforms, PR-A (81kDa) and PR-B (116kDa) . Expression of progesterone receptor has been suggested to reflect a intact estrogen regulatory machinery and therefore predict better clinical response to endocrine therapy than ER alone.

• Specifications:

  The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human Progesterone Receptor protein was used as the immunogen for this antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  PGSR17

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the Progesterone Receptor antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Progesterone Receptor antibody is available for research use only.

• Storage Conditions:

  Store the Progesterone Receptor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

• Applications Notes:

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Progesterone Receptor antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• CAS Number:

  9007-83-4

• Location:

  Nuclear, cytoplasmic

• Image Legend:

  IHC testing of FFPE human breast carcinoma with Progesterone Receptor antibody (clone PGSR17) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.