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Melan-A Antibody

CAT: 0800-V7016SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V7016SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
UniProt
Q16655
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the Melan-A antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
MRT3V
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the Melan-A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Melan-A antibody is available for research use only.
Storage Conditions
Store the Melan-A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the Melan-A antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Location
Cytoplasmic
Image Legend
IHC: Formalin-fixed, paraffin-embedded human melanoma stained with Melan-A antibody (clione MRT3V) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

UniProtKB · Q16655

Melanoma antigen recognized by T-cells 1

MAR1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q16655
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MLANA
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
MART-1
EC number
—
Processing
—
Secondary accessions
Q6ICU4
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Cellular component

Endoplasmic reticulumGolgi apparatusMembrane

PTM

Phosphoprotein

Domain

TransmembraneTransmembrane helix

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