HCG alpha Antibody / Luteinizing Hormone alpha

• Description:

  This antibody reacts with a protein of ~13kDa, identified as alpha sub-unit of hCG. HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. HCG is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH) .

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

• UniProt:

  P01215

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant protein was used as the immunogen for this hCG alpha antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  CGA91-2

• Applications:

  IHC-P

• Purity:

  Protein G purified monoclonal antibody

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the hCG alpha antibody at 2-8oC.

• Limitations:

  This hCG alpha antibody is available for research use only.

• Storage Conditions:

  Store the hCG alpha antibody at 2-8°C.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Variations in protocols, secondaries and substrates may require the hCG alpha antibody to be titered for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, secreted

• Image Legend:

  IHC testing of FFPE human placenta stained with hCG alpha antibody (CGA91-2) .