PCNA Antibody

• Description:

  Proliferating cell nuclear antigen is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. [UniProt]

• Specifications:

  Flow cytometry: 1-2ug/10^6 cells, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)

• UniProt:

  P12004

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant proliferating cell nuclear antigen was used as the immunogen for the PCNA antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  PM441-1

• Applications:

  WB, IHC-P, FACS

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the PCNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This PCNA antibody is available for research use only.

• Storage Conditions:

  Store the PCNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Nuclear with some cytoplasmic

• Image Legend:

  Formalin-fixed, paraffin-embedded human tonsil stained with PCNA antibody (clone PM441-1) . FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.