CRYAB Antibody

• Description:

  Crystallins are the major proteins of the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. Crystallins are divided into alpha, beta and gamma families, and the beta- and gamma-crystallins also compose a superfamily. Crystallins usually contain seven distinct protein regions, including four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha-crystallins consist of three gene products, alpha-A-, alpha-B- and alpha-C-crystallin, which are members of the small heat shock protein family (HSP 20) . Alpha-crystallins act as molecular chaperones by holding denatured proteins in large soluble aggregates. However, unlike other molecular chaperones, alpha-crystallins do not renature these proteins. Expression of alpha-A-crystallin is restricted to the lens and defects of this gene cause the development of autosomal dominant congenital cataracts (ADCC) . The human alpha-B-crystallin gene product is expressed in many tissues, including lens, heart and skeletal muscle. Elevated expression of alpha-B-crystallin is associated with many neurological diseases, and a missense mutation in this gene has co-segregated in a family with a Desmin-related myopathy.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

• UniProt:

  P02511

• Host:

  Mouse

• Reactivity:

  Human, Rat

• Immunogen:

  A full length recombinant human protein was used as the immunogen for the CRYAB antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2c κ

• Clone:

  CPTC-CRYAB-1

• Applications:

  WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the CRYAB antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CRYAB antibody is available for research use only.

• Storage Conditions:

  Store the CRYAB antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, nuclear

• Image Legend:

  IHC staining of FFPE human breast tissue with CRYAB antibody (clone CPTC-CRYAB-1) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.