CD21 Antibody

• Description:

  Recognizes a protein of 140kDa, which is identified as the complement receptor 2 (CR2) /CD21. Its epitope is located in 5-8 short consensus repeats (SCRs) . This mAb is highly specific to CR2 and shows no cross-reaction with CR1. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.

• Specifications:

  ELISA (order BSA/sodium azide-free format for coating), Flow cytometry: 1-2ug/10^6 cells, Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P20023

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 142-240 from the human protein was used as the immunogen for the CD21 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  CR2/2754

• Applications:

  ELISA, FACS, WB, IF, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the CD21 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD21 antibody is available for research use only.

• Storage Conditions:

  Store the CD21 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• CAS Number:

  9007-83-4

• Location:

  Cell surface

• Image Legend:

  Western blot testing of human Raji cell lysate with CD21 antibody (clone CR2/2754) .