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CD5 Antibody

CAT: 0800-V3961-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V3961-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a 67kDa transmembrane protein which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-) . Anti-CD5 does not react with granulocytes or monocytes.
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P06127
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 269-366 from the human protein was used as the immunogen for this CD5 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
CD5/2416
Applications
FACS, WB, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the CD5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD5 antibody is available for research use only.
Storage Conditions
Store the CD5 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The optimal dilution of the CD5 antibody for each application should be determined by the researcher.
Location
Cell surface
Image Legend
IHC staining of FFPE human tonsil with CD5 antibody (clone CD5/2416) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

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