Products for Research Use Only
RegisterLogin

ZAP70 Antibody

CAT: 0800-V3942-100UGSize: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0800-V3942-100UGSize:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, ELISA: 2-4 µg/mL (order BSA/azide-free format), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P43403
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 247-382 from the human protein was used as the immunogen for the ZAP70 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
ZAP70/2035
Applications
FACS, IF, ELISA, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the ZAP70 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This ZAP70 antibody is available for research use only.
Storage Conditions
Store the ZAP70 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the ZAP70 antibody should be determined by the researcher.
Location
Cytoplasmic, cell surface
Image Legend
IHC testing of FFPE human tonsil tissue with ZAP70 antibody (clone ZAP70/2035) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

UniProtKB · P43403

Tyrosine-protein kinase ZAP-70

ZAP70_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P43403
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ZAP70
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.7.10.2
Processing
—
Secondary accessions
A6NFP4, Q6PIA4, Q8IXD6, Q9UBS6
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondPhosphoproteinUbl conjugation

Biological process

Adaptive immunityImmunity

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingNucleotide-binding

Cellular component

Cell membraneCytoplasmMembrane

Disease

Disease variantSCID

Molecular function

KinaseTransferaseTyrosine-protein kinase

Domain

RepeatSH2 domain

Related Products

CatalogName
V7816-100UGGlycoprotein 2 Antibody / GP2 / ZAP75
V3873SAF-100UGGlycoprotein 2 Antibody / GP2 / ZAP75
V7816-20UGGlycoprotein 2 Antibody / GP2 / ZAP75
V3872-20UGGP2 Antibody / Glycoprotein 2 / ZAP75
V3333-20UGGP2 Antibody / Glycoprotein 2 / ZAP75
V3333-100UGGP2 Antibody / Glycoprotein 2 / ZAP75
V4410-100UGGlycoprotein 2 Antibody / GP2 / ZAP75
V3872SAF-100UGGP2 Antibody / Glycoprotein 2 / ZAP75
V4402-100UGGlycoprotein 2 Antibody / GP2 / ZAP75
V4410-20UGGlycoprotein 2 Antibody / GP2 / ZAP75

Alternative Products

CatalogName
GTR17857109ZAP70 Antibody

Popular Products