BOB1 Antibody

• Description :

  Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. [UniProt]

• Specifications :

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt :

  Q16633

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  A portion of amino acids 148-255 from the human protein was used as the immunogen for the BOB1 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2b κ

• Clone :

  PD2AF1-1

• Applications :

  IHC-P, WB

• Purity :

  Protein G affinity

• Format :

  Purified

• Buffer :

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution :

  Store the BOB1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This BOB1 antibody is available for research use only.

• Storage Conditions :

  Store the BOB1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes :

  The stated application concentrations are suggested starting points. Titration of the BOB1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Location :

  Nuclear

• Image Legend :

  IHC testing of FFPE human spleen with BOB-1 antibody (clone PD2AF1-1) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.