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BOB1 Antibody

CAT: 0800-V3915-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V3915-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. [UniProt]
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q16633
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 148-255 from the human protein was used as the immunogen for the BOB1 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
PD2AF1-1
Applications
IHC-P, WB
Purity
Protein G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the BOB1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This BOB1 antibody is available for research use only.
Storage Conditions
Store the BOB1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the BOB1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear
Image Legend
IHC testing of FFPE human spleen with BOB-1 antibody (clone PD2AF1-1) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Product Datasheet
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