BOB1 Antibody

• Description :

  BOB1, also called POU2AF1, expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB1. Conversely, only some cases of classical Hodgkin lymphoma show BOB1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells. Expression of BOB1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia. B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.

• Specifications :

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt :

  Q16633

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  A portion of amino acids 148-255 from the human protein was used as the immunogen for the BOB1 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2b κ

• Clone :

  BOB1/2425

• Applications :

  WB, IHC-P

• Purity :

  Protein G affinity

• Format :

  Purified

• Buffer :

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution :

  Store the BOB1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This BOB1 antibody is available for research use only.

• Storage Conditions :

  Store the BOB1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes :

  Optimal dilution of the BOB1 antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Location :

  Nuclear

• Image Legend :

  Western blot testing of human Raji cell lysate with BOB1 antibody (clone BOB1/2425) . Predicted molecular weight: ~28 kDa (unmodified), 35-40 kDa (ubiquitinated) .