CD40 Antibody

• Description :

  Cluster of differentiation 40, CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. [Wiki]

• Specifications :

  Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt :

  P25942

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  A portion of amino acids 29-107 from the human protein was used as the immunogen for the CD40 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2b κ

• Clone :

  CDLA40-1

• Applications :

  IHC-P, FACS

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution :

  Store the CD40 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This CD40 antibody is available for research use only.

• Storage Conditions :

  Store the CD40 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes :

  The stated application concentrations are suggested starting points. Titration of the CD40 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Location :

  Cell surface, cytoplasmic

• Image Legend :

  IHC testing of human tonsil with CD40 antibody (clone CDLA40-1) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.