CD40 Antibody

• Description:

  Cluster of differentiation 40, CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. [Wiki]

• Specifications:

  Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P25942

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 29-107 from the human protein was used as the immunogen for the CD40 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  CDLA40-1

• Applications:

  IHC-P, FACS

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the CD40 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD40 antibody is available for research use only.

• Storage Conditions:

  Store the CD40 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the CD40 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic

• Image Legend:

  IHC testing of human tonsil with CD40 antibody (clone CDLA40-1) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.