TCF4 Antibody

• Description:

  Recognizes a protein of 71kDa, identified as Transcription Factor 4 (TCF4) . It is a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome.

• Specifications:

  ELISA: 2-4 µg/mL (order BSA/azide-free format), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P15884

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 365-671 from the human protein was used as the immunogen for this TCF4 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  TCF4/1705

• Applications:

  ELISA, WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the TCF4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This TCF4 antibody is available for research use only.

• Storage Conditions:

  Store the TCF4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the TCF4 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nuclear

• Image Legend:

  Western blot testing of human 1) HeLa and 2) HepG2 cell lysate with TCF4 antibody (clone TCF4/1705) . Predicted molecular weight ~71 kDa.