TROP2 Antibody / TACSTD2

• Description:

  This intronless gene encodes a carcinoma-associated antigen defined by the monoclonal antibody GA733. This antigen is a member of a family including at least two type I membrane proteins. It transduces an intracellular calcium signal and acts as a cell surface receptor. [Wiki]

• Specifications:

  Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P09758

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 31-274 were used as the immunogen for the TROP2 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  TCSR2-1

• Applications:

  IHC-P, IF

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the TROP2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This TROP2 antibody is available for research use only.

• Storage Conditions:

  Store the TROP2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the TROP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  IHC testing of FFPE human colon carcinoma with TROP2 antibody (clone TCSR2-1) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.