CD25 Antibody

• Description:

  Recognizes a protein of 55 kDa, identified as CD25. It is expressed on activated T- and B-cells and activated monocytes/macrophages. With respect to lymphomas, CD25 is present on malignant cells of Hodgkins disease, HTLV-1+ adult T-cell leukemia, cutaneous T-cell lymphoma, and hair cell leukemia. Increased levels of soluble CD25 are observed in the leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 molecule alone appears to function as a low affinity receptor and associates with CD122 (IL-2R b-chain, p75) and CD132 (common g-chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. This mAb recognizes the epitope B, which is located at residue 3-104 of CD25 and does not block IL-2 binding to CD25.

• Specifications:

  Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 min at RT

• UniProt:

  P01589

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 42-183 was used as the immunogen for the CD25 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  IL2RA/2395

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the CD25 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD25 antibody is available for research use only.

• Storage Conditions:

  Store the CD25 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD25 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, Cytoplasmic

• Image Legend:

  IHC testing of FFPE human tonsil tissue with CD25 antibody (clone IL2RA/2395) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.