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AKT1 Antibody

CAT: 0800-V3798-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V3798-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. [UniProt]
CAS Number
9007-83-4
Specifications
IHC (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P31749
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 85-189 from the human protein was used as the immunogen for the AKT1 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
RASTPK-1
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the AKT1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This AKT1 antibody is available for research use only.
Storage Conditions
Store the AKT1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the AKT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nucleus, plasma membrane, cytoplasm
Image Legend
IHC testing of FFPE human pancreas with AKT1 antibody (clone RASTPK-1) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

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