CD21 Antibody

• Description :

  CD21/CR2 is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. This mAb is highly specific to CR2 and shows no cross-reaction with CR1.

• Specifications :

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

• UniProt :

  P20023

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  A portion of amino acids 44-196 were used as the immunogen for the CD21 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  CDLA21-1

• Applications :

  IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution :

  Store the CD21 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This CD21 antibody is available for research use only.

• Storage Conditions :

  Store the CD21 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes :

  The stated application concentrations are suggested starting points. Titration of the CD21 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Location :

  Cell surface

• Image Legend :

  IHC testing of FFPE human tonsil tissue with CD21 antibody (clone CDLA21-1) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.