Recombinant Calponin Antibody

• Description:

  Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction.

• Specifications:

  Western blot: 0.5-1 µg/mL, Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

• UniProt:

  P51911

• Host:

  Mouse

• Reactivity:

  Human, Rat

• Immunogen:

  Recombinant human protein was used as the immunogen for the recombinant Calponin antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG1 k

• Clone:

  RCNN1/832

• Type:

  Recombinant

• Applications:

  WB, FACS, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the recombinant Calponin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This recombinant Calponin antibody is available for research use only.

• Storage Conditions:

  Store the recombinant Calponin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9000-83-3

• Location:

  Cytoplasmic

• Image Legend:

  IHC testing of FFPE human uterus stained with recombinant Calponin antibody (clone rCNN1/832) . Required HIER: boil tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 min.