Calponin Antibody

CAT: 0800-V2464-100UGSize: 100 µgDry Ice: NoHazardous: No

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CAT#:0800-V2464-100UGSize:100 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this mAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P51911

Host

Mouse

Reactivity

Human

Immunogen

Crude human uterus extract was used as the immunogen for the Calponin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

SPM169

Applications

FACS, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Calponin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Calponin antibody is available for research use only.

Storage Conditions

Store the Calponin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

Immunofluorescent staining of PFA-fixed human K562 cells with Calponin antibody (clone SPM169, green) and Reddot nuclear stain (red) .

UniProtKB · P51911

Calponin-1

CNN1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P51911
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: CNN1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: —
Secondary accessions: B2R868, B4DUX6, O00638, Q15416, Q8IY93, Q99438

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Molecular function

Actin-bindingCalmodulin-binding

Coding sequence diversity

Alternative splicing

PTM

Phosphoprotein

Domain

Repeat

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Alternative Products

Catalog	Name
DF7671-01	Calponin Antibody