Recombinant MUC-1 Antibody

• Description:

  This mAb reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The dominant epitope of this MAb involves both amino acids as well as sugar moieties. Neuraminidase treatment destroys the antigen. It is a very good tracer agent in CA15.3 assays. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up-regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion.

• Specifications:

  The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• UniProt:

  P15941

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Human milk fat globule membranes were used as the immunogen for this recombinant MUC-1 antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  RMUC1/960

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

• Reconstitution:

  Store the recombinant MUC-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This recombinant MUC-1 antibody is available for research use only.

• Storage Conditions:

  Store the recombinant MUC-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

• Applications Notes:

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant MUC-1 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• CAS Number:

  9000-83-3

• Location:

  Cytoplasmic and cell surface

• Image Legend:

  IHC testing of FFPE human ovarian carcinoma with recombinant MUC-1 antibody (clone rMUC1/960) . Required HIER: boil sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.