CDH16 Antibody / Cadherin 16

• Description:

  This MAb recognizes a protein of 130kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport.It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells.Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels.Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.

• Specifications:

  ELISA: order unconjugated format for coating, Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 minutes at RT

• UniProt:

  O75309

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 371-507 from the human protein was used as the immunogen for the CDH16 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  CDH16/2125

• Applications:

  ELISA, WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Biotin Conjugate

• Buffer:

  0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the CDH16 antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).

• Limitations:

  This CDH16 antibody is available for research use only.

• Storage Conditions:

  Store the CDH16 antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term) .

• Formulation:

  0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CDH16 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface with some cytoplasmic

• Image Legend:

  IHC testing of FFPE human kidney with biotinylated CDH16 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.