Products for Research Use Only

Recombinant CD43 Antibody

CAT: 0800-V3653-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V3653-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43 [Workshop IV]. Epitope of mAb Bra7G is clearly different from that of mAb rSPN/839, called 'b' as opposed to 'a' for rSPN/839. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
CAS Number
9000-83-3
Specifications
Flow cytometry: 1-2ug/10^6 cells, Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P16150
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the recombinant CD43 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RSPN/839
Type
Recombinant
Applications
FACS, WB, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the recombinant CD43 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant CD43 antibody is available for research use only.
Storage Conditions
Store the recombinant CD43 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cell surface
Image Legend
Western blot testing of human K562 cell lysate with recombinant CD43 antibody (clone rSPN/839) . Expected molecular weight: 45-135 kDa depending on glycosylation level.

UniProtKB · P16150

Leukosialin

LEUK_HUMAN · Homo sapiens

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Primary accession
P16150
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SPN
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A8K9B1
Protein keywords

Cellular component

Cell projectionMembraneNucleus

Technical term

Direct protein sequencingProteomics identificationReference proteome

PTM

GlycoproteinPhosphoproteinUbl conjugation

Domain

SignalTransmembraneTransmembrane helix
Product Datasheet
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