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Recombinant CD44v4 Antibody

CAT: 0800-V3590-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V3590-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes an epitope encoded by exon v4 on the variant portion of human CD44. The CD44 molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. It is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA. The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P16070
Host
Mouse
Reactivity
Human
Immunogen
An amino acid sequence from the variant 4 domain of CD44 was used as the immunogen for the recombinant CD44v4 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG2a κ
Clone
RCD44v4/1219
Type
Recombinant
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the recombinant CD44v4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant CD44v4 antibody is available for research use only.
Storage Conditions
Store the recombinant CD44v4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cell surface, cytoplasmic
Image Legend
IHC testing of FFPE human cervical squamos cell carcinoma with recombinant CD44v4 antibody (clone rCD44v4/1219) . Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA buffer for 10-20 min.
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