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PHB Antibody / Prohibitin

CAT: 0800-V3518SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V3518SAF-100UGSize:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes a protein of 30kDa which is identified as Prohibitin (PHB), an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although PHB mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. It is an ideal mitochondrial marker. It shows anti-proliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P35232
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human partial protein corresponding to amino acids 167-261 was used as the immunogen for the PHB antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
PHB/1882
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the PHB antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This PHB antibody is available for research use only.
Storage Conditions
Store the PHB antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the PHB antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Location
Cytoplasmic
Image Legend
Western blot testing of human 1) A549, 2) K562, 3) A431 and 4) MCF7 cell lysate with PHB antibody (clone PHB/1882) . Expected molecular weight ~30 kDa.

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