DOG1 Antibody

• Description :

  Expression of DOG-1 protein is elevated in the gastrointestinal stromal tumors (GIST s), c-kit signaling-driven mesenchymal tumors of the GI tract. DOG-1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG-1 has been reported in 97.8 percent of scorable GIST s, including all c-kit negative GIST s. Overexpression of DOG-1 has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG1 and c-kit in GIST s is nearly identical: 94.4% vs. 94.7%.

• Specifications :

  The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• UniProt :

  Q5XXA6

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  A recombinant human protein corresponding to amino acids 2-101 of human DOG1 was used as the immunogen for this DOG1 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2b κ

• Clone :

  DG1/1486

• Applications :

  IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

• Reconstitution :

  Store the DOG1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This DOG1 antibody is available for research use only.

• Storage Conditions :

  Store the DOG1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

• Applications Notes :

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the DOG1 antibody to be titered up or down for optimal performance.

• CAS Number :

  9007-83-4

• Location :

  Cell Surface and Cytoplasmic

• Image Legend :

  IHC testing of FFPE human gastrointestinal stromal tumor (GIST) with DOG1 antibody (clone DG1/1486) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.