CFTR Antibody

• Description :

  Cystic fibrosis transmembrane conductance regulator (CFTR) is involved in the transport of chloride ions. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the SLC4A7 transporter. Can inhibit the chloride channel activity of ANO1. Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation. [UniProt]

• Specifications :

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt :

  P13569

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  A partial recombinant protein corresponding to amino acids 258-385 from the human protein was used as the immunogen for this CFTR antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2b κ

• Clone :

  CFTR/1785

• Applications :

  IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1X PBS, pH 7.4

• Reconstitution :

  Store the CFTR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This CFTR antibody is available for research use only.

• Storage Conditions :

  Store the CFTR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes :

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CFTR antibody to be titered up or down for optimal performance.

• CAS Number :

  9007-83-4

• Location :

  Cell surface, cytoplasmic

• Image Legend :

  IHC testing of FFPE human pancreas with CFTR antibody (clone CFTR/1785) . HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.