P-Cadherin Antibody / CDH3

• Description:

  Recognizes a protein of 116kDa, identified as P-Cadherin, or Cadherin 3 or CDH3. It is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congenital hypotrichosis with juvenile macular dystrophy.

• Specifications:

  Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 0.5-1 µg/mL

• UniProt:

  P22223

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Membrane from trypsinized A431 cells was used as the immunogen for this P-Cadherin antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  6A9

• Applications:

  FACS, IF, WB

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the P-Cadherin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This P-Cadherin antibody is available for research use only.

• Storage Conditions:

  Store the P-Cadherin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the P-Cadherin antibody to be titered up or down for optimal performance.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic

• Image Legend:

  Western blot testing of human A431 cell lysate with P-Cadherin antibody (clone 6A9) . Expected molecular weight: ~91 kDa (unmodified), 100~130 kDa (glycosylated) .