EpCAM Antibody / Extracellular domain

• Description:

  EpCAM (Epithelial Cell Adhesion Molecule), also known as CD326, plays a crucial role in various cellular processes and has been linked to the development and progression of cancer. EpCAM is a transmembrane glycoprotein that is expressed in high levels on the surface of epithelial cells. In cancer cells, the expression of EpCAM is often upregulated, leading to increased cell proliferation, invasion, and metastasis. Studies have shown that high levels of EpCAM are associated with poor prognosis in various types of cancer, including breast, colon, and lung cancer. The overexpression of EpCAM in cancer cells makes it an attractive target for diagnostic and therapeutic purposes. Researchers are exploring the use of EpCAM as a biomarker for early cancer detection through blood tests and imaging techniques. In addition, EpCAM-targeted therapies, such as monoclonal antibodies and immunotherapies, are being developed to specifically target and kill cancer cells that overexpress EpCAM.

• Specifications:

  ELISA: order BSA/sodium azide-free format for coating, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 1-2 µg/mL

• UniProt:

  P16422

• Host:

  Mouse

• Reactivity:

  Human, Dog, Cat, Guinea Pig

• Immunogen:

  A human partial recombinant protein corresponding to amino acids 100-224 (extracellular domain) was used as the immunogen for the EpCAM antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG

• Clone:

  EGP40/1384

• Applications:

  ELISA, IF, IHC-P, WB

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This EpCAM antibody is available for research use only.

• Storage Conditions:

  Store the EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Titering of the EpCAM antibody may be required for optimal performance.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic

• Image Legend:

  IHC testing of FFPE human ovarian carcinoma and EpCAM antibody (clone EGP40/1384) . Required HIER: steam sections in pH6 citrate buffer for 10-20 min.