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DOG 1 Antibody

CAT: 0800-V3335-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V3335-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
DOG 1 is expressed in the human gastrointestinal epithelium and is highly expressed in the gastrointestinal interstitial cells of Cajal, where it plays an important role in epithelial chloride secretion mediating intestinal motility. [Wiki]
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q5XXA6
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human protein corresponding to amino acids 2-101 of human DOG1/ANO1 was used as the immunogen for this DOG 1 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
DG1/1484
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the DOG 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This DOG 1 antibody is available for research use only.
Storage Conditions
Store the DOG 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the DOG 1 antibody to be titered up or down for optimal performance.
Location
Cell Surface and Cytoplasmic
Image Legend
IHC testing of FFPE human gastrointestinal stromal tumor (GIST) with DOG 1 antibody (DG1/1484) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.

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