Desmocollin 2/3 Antibody

• Description:

  Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. The desmosome is subdivided into two regions. The plaque region lies adjacent to the plasma, and is believed to contain molecules that attach the intermediate filament cytoskeleton to the desmosome. The core region is composed of transmembrane glycoproteins that are thought to mediate cell-cell adhesion. Desmogleins and desmocollins are the main desmosomal transmembrane proteins. These desmosomal glycoproteins belong to the members of the cadherin family of adhesion molecules. Three different isoforms of both desmogleins and desmocollins have been identified, named as desmoglein 1-3 and desmocollins. Desmosomal cadherins showed differentiation-specific expression in the human epidermis, although the functional significance of this differential expression is not fully understood. Desmocollin-1 can be found in the upper layers. The expression of Desmocollin-2 varies in the basal and suprabasal layers. And Desmocollin-3 is expressed more evenly throughout the suprabasal layers.

• Specifications:

  Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  Q02487

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A recombinant protein corresponding to the extracellular domain of human Desmocollin 2 was used as the immunogen for the Desmocollin 2/3 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  7G6

• Applications:

  FACS, IF, WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the Desmocollin 2/3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Desmocollin 2/3 antibody is available for research use only.

• Storage Conditions:

  Store the Desmocollin 2/3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the Desmocollin 2/3 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface

• Image Legend:

  IHC testing of FFPE human skin with Desmocollin 2/3 antibody (clone 7G6) . Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min.