CD61 Antibody / ITGB3 / Integrin beta 3

• Description:

  Reacts with human CD61, also called Integrin beta 3 (GPIIIa, vitronectin receptor beta chain) . It associates with the αV-chain (CD51) to form vitronectin receptor, or with the αIIb-chain (CD41) to form the GpIIb/GpIIIa complex (CD41/CD61) . The CD41/CD61 complex appears early in megakaryocyte maturation. The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen, fibronectin, vitronectin and thrombospondin. It plays a central role in platelet activation and aggregation. The CD51/CD61 is implicated in tumor metastasis and adenoviral infection. The antibody detects platelets in smears of blood and bone marrow, as well as megakaryocytes in frozen sections and cell smears. The antibody is useful for classification of megakaryoblastic leukemia.

• Specifications:

  Immunohistochemistry (Frozen & FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P05106

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Phytohemagglutinin (PHA) -stimulated peripheral blood cells were used as the immunogen for the CD61 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  Y2/51

• Applications:

  IHC

• Purity:

  Protein G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the CD61 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD61 antibody is available for research use only.

• Storage Conditions:

  Store the CD61 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Titration of the CD61 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic

• Image Legend:

  IHC testing of FFPE human spleen tissue with CD61 antibody (clone Y2/51) . Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.