Desmin Antibody

• Description :

  Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain α -helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles. Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.

• Specifications :

  Immunofluorescence: 1-2 µg/mL, Flow cytometry: 0.5-1ug/10^6 cells, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt :

  P17661

• Host :

  Mouse

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Recombinant human Desmin was used as the immunogen for the Desmin antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  DES/1711

• Applications :

  IF, FACS, WB, IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution :

  Store the Desmin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This Desmin antibody is available for research use only.

• Storage Conditions :

  Store the Desmin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes :

  Optimal dilution of the Desmin antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Location :

  Cytoplasmic

• Image Legend :

  IHC testing of FFPE human uterus with Desmin antibody (clone DES/1711) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.