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Myogenin Antibody

CAT: 0800-V3153-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V3153-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6) . MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.
CAS Number
9007-83-4
Specifications
ELISA (order BSA/sodium azide-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P15173
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids 138-158 from the rat protein were used as the immunogen for this Myogenin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
F5D
Applications
ELISA, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Myogenin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Myogenin antibody is available for research use only.
Storage Conditions
Store the Myogenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Nuclear
Image Legend
IHC: Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Myogenin antibody (F5D)

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