BrdU Antibody

• Description:

  It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

• Specifications:

  The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• UniProt:

  Not Applicable

• Host:

  Mouse

• Reactivity:

  All Species Expected

• Immunogen:

  Bromodeoxyuridine was used as the immunogen for this BrdU antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  BU20a

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

• Reconstitution:

  Store the BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This BrdU antibody is available for research use only.

• Storage Conditions:

  Store the BrdU antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

• CAS Number:

  9007-83-4

• Location:

  Nuclear

• Image Legend:

  IHC: Formalin-fixed, paraffin-embedded mouse small intestine stained with BrdU antibody (BU20a) .