BrdU Antibody

CAT: 0800-V3112IHC-7MLSize: 7 mLDry Ice: NoHazardous: No
CAT#:0800-V3112IHC-7MLSize:7 mL
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
CAS Number
9007-83-4
Specifications
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
UniProt
Not Applicable
Host
Mouse
Reactivity
All Species Expected
Immunogen
Bromodeoxyuridine conjugated to hemocyanine was used as the immunogen for the BrdU antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
85-2C8
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Reconstitution
Store the BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This BrdU antibody is available for research use only.
Storage Conditions
Store the BrdU antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*
Location
Nuclear
Image Legend
IHC: Formalin-fixed, paraffin-embedded mouse small intestine stained with BrdU antibody (85-2C8) .

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