BrdU Antibody

• Description:

  It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

• Specifications:

  Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

• UniProt:

  Not Applicable

• Host:

  Mouse

• Reactivity:

  All Species Expected

• Immunogen:

  Bromodeoxyuridine conjugated to hemocyanine was used as the immunogen for the BrdU antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  85-2C8

• Applications:

  FACS, IF, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This BrdU antibody is available for research use only.

• Storage Conditions:

  Store the BrdU antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Nuclear

• Image Legend:

  IHC: Formalin-fixed, paraffin-embedded mouse small intestine stained with BrdU antibody (85-2C8) .