Anti-Mitochondrial Antibody

• Description:

  This mAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.

• Specifications:

  ELISA (order BSA/sodium azide-free format for coating), Immunofluorescence: 0.5-1 µg/mL, Western blot: 0.25-0.5 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

• UniProt:

  Not Known

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A semi-purified mitochondrial preparation was used as the immunogen for the anti-Mitochondrial antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  AE-1

• Applications:

  ELISA, IF, WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the anti-Mitochondrial antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This anti-Mitochondrial antibody is available for research use only.

• Storage Conditions:

  Store the anti-Mitochondrial antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic (Mitochondria)

• Image Legend:

  IHC: Formalin-fixed, paraffin-embedded human pancreas stained with anti-Mitochondrial antibody (clone AE-1) .