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Anti-Mitochondrial Antibody

CAT: 0800-V3111-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V3111-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This mAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.
CAS Number
9007-83-4
Specifications
ELISA (order BSA/sodium azide-free format for coating), Immunofluorescence: 0.5-1 µg/mL, Western blot: 0.25-0.5 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT
UniProt
Not Known
Host
Mouse
Reactivity
Human
Immunogen
A semi-purified mitochondrial preparation was used as the immunogen for the anti-Mitochondrial antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
AE-1
Applications
ELISA, IF, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the anti-Mitochondrial antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This anti-Mitochondrial antibody is available for research use only.
Storage Conditions
Store the anti-Mitochondrial antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic (Mitochondria)
Image Legend
IHC: Formalin-fixed, paraffin-embedded human pancreas stained with anti-Mitochondrial antibody (clone AE-1) .

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