Testosterone Antibody

• Description:

  This mAb is highly specific to testosterone. Its affinity constant for testosterone is ~10^10 M-1. In competitive binding immunoassay, it reacts with testosterone 100%, 11-beta-hydroxy testosterone 3.3%, 17-alpha-methyl testosterone.

• Specifications:

  ELISA (order BSA/sodium azide-free format for coating), Radioimmnoassay (RIA), Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT

• UniProt:

  Not Applicable

• Host:

  Mouse

• Reactivity:

  All Species Expected

• Immunogen:

  Testosterone 3 CMO conjugated to BSA was used as the immunogen for the Testosterone antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  4E1G2

• Applications:

  ELISA, RIA, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the Testosterone antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Testosterone antibody is available for research use only.

• Storage Conditions:

  Store the Testosterone antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the Testosterone antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Secreted and cytoplasmic

• Image Legend:

  IHC testing of FFPE human testicular carcinoma with Testosterone antibody (clone 4E1G2) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.