CLIP Antibody

• Description :

  Recognizes proteins of 33, 35 and 41kDa, which are identified as various isoforms of CD74/CLIP. Its epitope is localized in the extracellular domain of CD74. CD74/CLIP is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. The molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes. CD74/CLIP is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, and dendritic cells and by various types of carcinomas. It is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74/CLIP stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas and has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+) .

• Specifications :

  Western blot: 1-2 µg/mL

• UniProt :

  P04233

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Recombinant full-length human protein was used as the immunogen for the CLIP antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  CLIP/1133

• Applications :

  WB

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution :

  Store the CLIP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This CLIP antibody is available for research use only.

• Storage Conditions :

  Store the CLIP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes :

  Optimal dilution of the CLIP antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Location :

  Cell surface and paranuclear globular

• Image Legend :

  Western blot analysis of Raji cell lysate using CLIP antibody (CLIP/1133) . Expected molecular weight: 33-43 kDa.