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CD30 Antibody Cocktail

CAT: 0800-V2994S-0.5MLSize: 0.5 mLDry Ice: NoHazardous: No
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CAT#:0800-V2994S-0.5MLSize:0.5 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a single chain glycoprotein of 105/120kDa, identified as CD30/Ki-1. CD30 is synthesized as a 90kDa precursor, which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120kDa glycoprotein. In Hodgkin's disease, CD30/Ki-1 antigen is expressed by mononuclear-Hodgkin and multinucleated Reed-Sternberg cells. It is also expressed by the tumor cells of a majority of anaplastic large cell lymphomas as well as by a varying proportion of activated T and B cells. This mAb distinguishes large cell lymphomas derived from activated lymphoid cells from histiocytic malignancies and lymphomas derived from resting and precursor lymphoid cells or from anaplastic carcinomas. About one third of the Ki-1 positive lymphomas lack the leukocyte common antigen (CD45) .
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1:100-1:200 for 30 min at RT (1)
UniProt
P28908
Host
Mouse
Reactivity
Human
Immunogen
A Co cell line established from a patient with Hodgkin's disease of T-cell lineage (Ber-H2) and human recombinant protein (CD30/412) were used as the immunogen for the CD30 antibody cocktail.
Clonality
Monoclonal
Isotype
IgGs
Clone
Ber-H2 + CD30/412
Applications
IHC-P
Purity
Unpurified high titer supernatant
Format
Culture supernatant
Buffer
Bioreactor concentrate with 0.05% sodium azide
Reconstitution
Store the CD30 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD30 antibody cocktail is available for research use only.
Storage Conditions
Store the CD30 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
Bioreactor concentrate with 0.05% sodium azide
Applications Notes
Optimal dilution of the CD30 antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Location
Cell surface, cytoplasmic
Image Legend
IHC: Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with CD30 antibody (Ber-H2 + CD30/412) .

UniProtKB · P28908

Tumor necrosis factor receptor superfamily member 8

TNR8_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P28908
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
TNFRSF8
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
B1AN79, B9EGD9, D3YTD8, Q6P4D9
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative initiationAlternative splicing

Cellular component

Cell membraneCytoplasmMembrane

PTM

Disulfide bondGlycoproteinPhosphoprotein

Molecular function

Receptor

Domain

RepeatSignalTransmembraneTransmembrane helix
Product Datasheet
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