CD22 Antibody

• Description:

  Recognizes a protein of 130-140 kDa, identified as CD22 (also known as BL-CAM) . CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.

• Specifications:

  Flow cytometry: 0.5-1ug/10^6 cells

• UniProt:

  P20273

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Human Raji cells were used as the immunogen for the CD22 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  MYG13

• Applications:

  FACS

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the CD22 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD22 antibody is available for research use only.

• Storage Conditions:

  Store the CD22 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD22 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic

• Image Legend:

  SDS-PAGE Analysis of Purified, BSA-Free CD22 Antibody (clone MYG13) . Confirmation of Integrity and Purity of the Antibody.