Products for Research Use Only
RegisterLogin

WT1 Antibody

CAT: 0800-V2932-20UGSize: 20 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0800-V2932-20UGSize:20 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors. WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P19544
Host
Mouse
Reactivity
Human, Rat
Immunogen
A recombinant human protein was used as the immunogen for the WT1 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
WT1/857
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the WT1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This WT1 antibody is available for research use only.
Storage Conditions
Store the WT1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
WT1/857 mAb
Location
Nuclear, cytoplasmic
Image Legend
IHC testing of formalin-fixed, paraffin-embedded human testis stained with WT1 antibody (clone WT1/857) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Related Products

CatalogName
V4136-20UGWilms Tumor 1 Antibody / WT1
V4135-100UGWilms Tumor 1 Antibody / WT1
V5193-20UGWT1-associated protein Antibody / WTAP
V4136SAF-100UGWilms Tumor 1 Antibody / WT1
V5193SAF-100UGWT1-associated protein Antibody / WTAP
V4135SAF-100UGWilms Tumor 1 Antibody / WT1
V4136-100UGWilms Tumor 1 Antibody / WT1
V4135-20UGWilms Tumor 1 Antibody / WT1
V5193-100UGWT1-associated protein Antibody / WTAP
V8926-20UGRecombinant Wilms Tumor 1 Antibody / WT1

Alternative Products

CatalogName
MBS8529199-01H2B Antibody Antibody
MBS8513599-01CD11b Antibody Antibody
MBS850197-01Antibody to IGFBP2 Antibody
DF8746-01ASAP1 antibody Antibody
E36403ZNF98 Antibody Antibody
MBS9403836-01SZT2 Antibody Antibody
GWB-250026Lck antibody Antibody
E8M1007-4HSP60 Antibody Antibody
LSMab09882anti-Antibody^Polyclonal antibody control antibody
GWB-5C6547MAD1L1 antibody [9B10] Antibody

Popular Products