CD90 Antibody / Thy1

• Description:

  Recognizes a protein of 18-35 kDa, identified as CD90 (also known as Thy1) . CD90 is a member of the immunoglobulin superfamily. It may contribute to inhibition of proliferation/differentiation of hematopoietic stem cells and neuron memory formation in the CNS. It consists of a single Ig domain (112 amino acids; 25-35 kDa) inserted into the cell membrane via a GPI anchor. Expressed by hematopoietic stem cells and neurons in all species studied. Its highly expressed in connective tissue and various fibroblast and stromal cell lines, expressed on all thymocytes and peripheral T cells in mice, but in humans expressed only on small % fetal thymocytes, 10-40% of CD34+ cells in bone marrow, and <1% of CD3+CD4+ lymphocytes in peripheral circulation. It is also expressed by human lymph node HEV endothelium but not other endothelia. Lastly, it is expressed by a limited number of lymphoblastoid and leukemic cell lines

• Specifications:

  Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 0.5-1 µg/mL

• UniProt:

  P04216

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Human T-lymphoma cells were used as the immunogen for the CD90 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  AF-9

• Applications:

  FACS, IF

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the CD90 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD90 antibody is available for research use only.

• Storage Conditions:

  Store the CD90 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD90 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface

• Image Legend:

  SDS-PAGE Analysis of Purified, BSA-Free CD90 Antibody (clone AF-9) . Confirmation of Integrity and Purity of the Antibody.